Protocols

For S. pombe (including protocols for S. cerevisiae)
pombe basic
How to make media
Pombe media
Media with drug
Cerevisiae media
Gene tagging, disruption
transformation (pombe)
transformation (cerevisiae)
Overexpression and depeletion of a protein using nmt1 promoter
Protein extraction for SDS-PAGE / buffer recipe
Cds1 kinase assay
Immunoprecipitation / Immunoprecipitation with antibody conjugated beads
Chromatin Immunoprecipitation (ChIP) (2009 version including use of DMP) (2014 version including use of Chelex-100, qPCR, SYBR green)
ChIP cell collection
ChIP PCR
In situ chromatin binding assay
DAPI staining
Immunofluorescence Microscopy / buffer recipe
Two dimensional (2D) agarose gel electrophoresis of chromosomal DNA
Pulsed Field Gel Electrophoresis
Reverse Transcriptase PCR (RT-PCR) and quantitative PCR (qPCR)

For mammalian tissue cultured cells
Culture media
Synchronization of Mammalian Cell Culture
b-gal assay
Protein extraction for SDS-PAGE
Immunoprecipitation
Immunofluorescence Microscopy and in situ fractionation
Cell fractionation with Triton X-100
Chromatin Immunoprecipitation (linked to PubMed)
Chromosome spreads
Pulsed-field Gel Electrophoresis

General Protocols
Restriction enzyme digetion
Subcloning
E. coli transformation & competent cells
Glycerol stock
SDS-PAGE / gel recipe
Western blotting
Southern blotting
Northern blotting
Site directed mutagenesis
lambda phopphatase treatment of immunoprecipitated proteins
Purification of GST-fusion proteins from Bacteria BL21(DE3) cells
Purification of His-tagged proteins from Bacteria BL21(DE3) cells

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Copy right (C) 2004 -2014
Eishi Noguchi